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GSK3 beta Mouse mAb

261206

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261206
Product Name:   Anti-GSK3 beta Mouse Monoclonal Antibody
Synonyms:   GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
UNIPROT ID:   P49841
Background:    Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis.
Immunogen:    Purified recombinant fragment of human GSK3B (AA: 2-159) expressed in E. Coli.
Applications:    WB,IHC-P,FC
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 FC: 1/50-1/100
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    7D8A6
MW:    Calculated MW: 47 kDa; Observed MW: 47 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded rectum cancer tissues using GSK3B antibody with DAB staining.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Flow Cytometry analysis of NIH/3T3 cells stained with GSK3B antibody (green) and negative control (red).

Immunohistochemistry analysis of paraffin-embedded esophageal cancer tissues using GSK3B antibody with DAB staining.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of GSK3B antibody in Hela, 3T3, C6, rat Brain lysates using GSK3B antibody.
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