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NSE (4C7) Mouse mAb

261224

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261224
Product Name:   Anti-NSE (4C7) Mouse Monoclonal Antibody
Synonyms:   ENO2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE
UNIPROT ID:   P09104
Background:    ENO2 an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Immunogen:    Synthetic Peptide of NSE
Applications:    WB,IHC-F,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    4C7-4F10-10F9
MW:    Calculated MW: 47 kDa; Observed MW: 47 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human small cell carcinoma of lung tissue using NSE antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Enolase2 in COS7, C6 and Hela lysates using Enolase2 antibody.

Western blot analysis of NSE (4C7) in Enolase (4C7) in CHO-K1, rat brain, C6, 3T3, Hela lysates using Enolase (4C7) antibody.

Immunocytochemistry analysis of NSE (4C7) (green) in Hela using NSE (4C7) antibody ,and DAPI(blue)
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