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XRCC4 (7C10) Mouse mAb

261232

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261232
Product Name:   Anti-XRCC4 (7C10) Mouse Monoclonal Antibody
Synonyms:   X ray repair cross complementing protein 4; DNA repair protein XRCC4; DNA double strand break repair
UNIPROT ID:   Q13426
Background:    Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Immunogen:    Synthetic Peptide of XRCC4
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    7C10-5H8-4F8
MW:    Calculated MW: 38 kDa; Observed MW: 38-45 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation analysis of XRCC4 in Hela lysates using XRCC4 antibody.

Immunohistochemistry analysis of paraffin-embedded XRCC4 in Human lung cancer using XRCC4 (7C10) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of XRCC4 (7C10) in XRCC4 in T47D, 3T3, COS7, C6 and Hela lysates using XRCC4 (7C10) antibody
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