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DiMethyl-Histone H3 (Lys27) (5F6) Mouse mAb

261236

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261236
Product Name:   Anti-DiMethyl-Histone H3 (Lys27) (5F6) Mouse Monoclonal Antibody
Synonyms:   H3K27me2; H3 histone; HIST1H3A; Histone cluster 1; H3a
UNIPROT ID:   P68431
Background:    H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Immunogen:    Synthetic Peptide of Histone H3 (Di Methyl Lys27)
Applications:    WB,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    5F6-6G6-6F9
MW:    Calculated MW: 15 kDa; Observed MW: 15 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Methylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    EpigeneticsyyyyyHistone methylation
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis ofDiMethyl-Histone H3 (Lys27) in HeLa cells using DiMethyl-Histone H3 (Lys27) (5F6) antibody.

Immunoprecipitation analysis of DiMethyl-Histone H3 (Lys27) (5F6) in Hela lysates using DiMethyl-Histone H3 (Lys27) (5F6) antibody.

Western blot analysis of DiMethyl-Histone H3 (Lys27) (5F6) in C6, 3T3, C2C12 lysates using DiMethyl-Histone H3 (Lys27) (5F6) antibody

Western blot analysis of DiMethyl-Histone H3 (Lys27) (5F6) in Hela, rat heart, mouse heart, HUVEC, Jurkat lysates using DiMethyl-Histone H3 (Lys27) (5F6) antibody.
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