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EGFR (5E9) Mouse mAb

261247

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261247
Product Name:   Anti-EGFR (5E9) Mouse Monoclonal Antibody
Synonyms:   EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
UNIPROT ID:   P00533
Background:    EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. . A single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation.
Immunogen:    Synthetic Peptide of EGFR
Applications:    WB,IP
Recommended Dilutions:    WB: 1/500-1/1000 IP: 1/20
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    5E9-10B8-8B9
MW:    Calculated MW: 134 kDa; Observed MW: 170 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation analysis of EGFR (5E9) in Hela lysates using EGFR (5E9) antibody

Western blot analysis of EGFR (5E9) in A431, Hela lysates using EGFR (5E9) antibody.

Western blot analysis of EGFR (5E9) in EGF Receptor/ErbB1 in Hela lysates using EGF Receptor/ErbB1 (5E9) antibody.
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