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eIF4A1 (6D7) Mouse mAb

261248

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261248
Product Name:   Anti-eIF4A1 (6D7) Mouse Monoclonal Antibody
Synonyms:   Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1
UNIPROT ID:   P60842
Background:    ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.
Immunogen:    Synthetic Peptide of eIF4A1
Applications:    WB,IHC-F,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    6D7-10D5-7H7
MW:    Calculated MW: 46 kDa; Observed MW: 46 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunofluorescence analysis of eIF4A1 (6D7) in Hela using eIF4A1 (6D7) antibody(Left) ,and DAPI (blue)

Western blot analysis of eIF4A1 in T47D, 3T3, COS7, C6 and Hela lysates using eIF4A1 antibody.

Western blot analysis of eIF4A1 (6D7) in CHO-K1, rat brain, C6, 3T3, Hela lysates using eIF4A (6D7) antibody.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using eIF4A1 (6D7) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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