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EGFR (3A9) Mouse mAb

261270

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261270
Product Name:   Anti-EGFR (3A9) Mouse Monoclonal Antibody
Synonyms:   EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
UNIPROT ID:   P00533
Background:    EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. . A single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation.
Immunogen:    Synthetic Peptide of EGFR
Applications:    WB,IHC-F,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    3A9-9D2-9F7
MW:    Calculated MW: 134 kDa; Observed MW: 170 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of EGFR (3A9) in Hela, A549 lysates using EGFR (3A9) antibody

Immunofluorescence analysis of EGFR in Hela using EGFR (3A9) antibody (Left) and DAPI (Right).

Western blot analysis of EGFR (3A9) in Hela lysates using EGFR (3A9) antibody

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using EGFR antibody High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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