Smad2/3 (6F7) Mouse mAb

261294

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

Add to cart

Download

Datasheet

MSDS

Product Description Reference citations(0) Related Products

Cat.#: N261294

Product Name: Anti-Smad2/3 (6F7) Mouse Monoclonal Antibody

Synonyms: SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

UNIPROT ID: P84022/Q15796

Background: Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 . Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses .

Immunogen: Recombinant Protein of Smad3

Applications: IHC-P,WB

Recommended Dilutions: WB: 1/500-1/1000 IHC: 1/50-1/100

Host Species: Mouse

Clonality: Mouse Monoclonal

Clone ID: -

MW: Calculated MW: 52 kDa; Observed MW: 52,60 kDa

Isotype: IgG

Purification: Affinity Purified

Species Reactivity: Human,Rat,Mouse

Conjugation: Unconjugated

Modification: Unmodified

Constituents: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Research Areas: Signal Transduction

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemical analysis of paraffin-embedded Human tonsils using Smad2/3 (6F7) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.	Immunohistochemistry analysis of paraffin-embedded Human Live rTissue using Smad2/3 (6F7) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Western blot analysis of Smad2/3 (6F7) in C6, MCF-7, Jurkat and 3T3 lysates using Smad2/3 (6F7) antibody

Reference citations

No data available

Product Targets

Product Categories

Smad2/3 (6F7) Mouse mAb

Vimentin mAb

TFF3 mAb

Transferrin mAb

survivin mAb

Contant Us