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LC3A (8F5) Mouse mAb

261327

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261327
Product Name:   Anti-LC3A (8F5) Mouse Monoclonal Antibody
Synonyms:   Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha
UNIPROT ID:   Q9H492
Background:    Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.
Immunogen:    Purified recombinant protein expressed in E.coli.
Applications:    WB,IHC-P
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    8F5-10H1-9A7
MW:    Calculated MW: 14 kDa; Observed MW: 14,16 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Rat,Mouse
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    AutophagyyyyyyAutophagosome
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human Heptacarcinoma Tissue using LC3A antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of LC3A (8F5)in Hela lysates, 3T3 lysates, rat Brain lysates using LC3A antibody.

Immunohistochemistry analysis of paraffin-embedded Human Prostate Carcinoma Tissue using LC3A antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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