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Hsp27 (9G1) Mouse mAb

261367

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261367
Product Name:   Anti-Hsp27 (9G1) Mouse Monoclonal Antibody
Synonyms:   HSPB1; HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27
UNIPROT ID:   P04792
Background:    Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway.
Immunogen:    Purified recombinant protein expressed in E.coli.
Applications:    WB,IHC-P
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    9G1-4D1-2G2
MW:    Calculated MW: 23 kDa; Observed MW: 27 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human Breast Caricnoma using HSP27 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Hsp27 (9G1) in Helalysates using Hsp27 (9G1) antibody.

Western blot analysis of Hsp27 (9G1) in K562, Hela lysates using HSP27 (9G1) antibody.
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