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GRP78 BiP (6H7) Mouse mAb

261378

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261378
Product Name:   Anti-GRP78 BiP (6H7) Mouse Monoclonal Antibody
Synonyms:   HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP
UNIPROT ID:   P11021
Background:    When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).
Immunogen:    Synthetic peptide conjugated to KLH.
Applications:    WB,IHC-P
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    6H7-4B1-9H4
MW:    Calculated MW: 72 kDa; Observed MW: 78 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Tags & Cell Markers
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemical analysis of paraffin-embedded Human tonsils using GRP78 BiP (6H7) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunohistochemistry analysis of paraffin-embedded Human Colon Caricnoma using GRP78 BiP (6H7) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of GRP78 BiP (6H7) in Jurkat, MCF-7, SKBR3, Hela lysates using GRP78 BiP (6H7) antibody

Immunocytochemistry analysis of GRP78 BiP in HeLa cells using GRP78 BiP (6H7) antibody.

Western blot analysis of GRP78 BiP (6H7) in CHO-K1, rat brain, C6, 3T3, Hela lysates using GRP78 BiP (6H7) antibody
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