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HP1 gamma (5G10) Mouse mAb

261398

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261398
Product Name:   Anti-HP1 gamma (5G10) Mouse Monoclonal Antibody
Synonyms:   CBX3; Chromobox protein homolog 3; HECH; Heterochromatin protein 1 homolog gamma; HP1 gamma; Modifier 2 protein
UNIPROT ID:   Q13185
Background:    Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Immunogen:    Purified recombinant human HP1-gamma protein fragments expressed in E.coli.
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    5G10-F7-A12
MW:    Calculated MW: 21 kDa; Observed MW: 21 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Mouse,Human,Monkey,Hamster,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of HP1 gamma (5G10) in HeLa using HP1 gamma antibody.

Western blot analysis of HP1gamma in Hela, 3T3, C6, COS7 and CHO-K1 lysates using HP1 gamma antibody.

Immunoprecipitation analysis of HP1 gamma (5G10) in Hela lysates using HP1gamma antibody.

Immunohistochemistry analysis of paraffin-embedded Prostate Cancer using HP1 gamma antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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