GRP78 BiP (4F11) Mouse mAb

261400

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: N261400

Product Name: Anti-GRP78 BiP (4F11) Mouse Monoclonal Antibody

Synonyms: HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP

UNIPROT ID: P11021

Background: When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).

Immunogen: Purified recombinant human BiP/GRP78 protein expressed in E.coli.

Applications: WB,ICC/IF,IP

Recommended Dilutions: WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20

Host Species: Mouse

Clonality: Mouse Monoclonal

Clone ID: 4F11-D5-C10

MW: Calculated MW: 72 kDa; Observed MW: 78 kDa

Isotype: IgG2b

Purification: Affinity Purified

Species Reactivity: Human,Mouse,Rat

Conjugation: Unconjugated

Modification: Unmodified

Constituents: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Research Areas: NeuroscienceyyyyyPrion disease

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunofluorescence analysis of GRP78 BiP (4F11) in Hela using BiP/GRP78 antibody.	Western blot analysis of GRP78 BiP (4F11) in 3T3, A549, C6 and Hela lysates using BiP/GRP78 antibody.
Immunoprecipitation analysis of GRP78 BiP (4F11) in Hela lysates using BiP/GRP78 antibody.Western blot analysis of BiP/GRP78 using BiP/GRP78 antibody.

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