GRP78 BiP (9E4) Mouse mAb

261401

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: N261401

Product Name: Anti-GRP78 BiP (9E4) Mouse Monoclonal Antibody

Synonyms: HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP

UNIPROT ID: P11021

Background: When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).

Immunogen: Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli.

Applications: WB,IHC-F,IHC-P,ICC/IF

Recommended Dilutions: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Host Species: Mouse

Clonality: Mouse Monoclonal

Clone ID: 9E4-2A7-H6

MW: Calculated MW: 72 kDa; Observed MW: 78 kDa

Isotype: IgG1

Purification: Affinity Purified

Species Reactivity: Human,Rat

Conjugation: Unconjugated

Modification: Unmodified

Constituents: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Research Areas: Tags & Cell Markers

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of GRP78 BiP (9E4) in HeLa using BiP/GRP78 (Cterminus) antibody.	Western blot analysis of BiP/GRP78 (Cterminus) in Hela, C6, Lncap and MDA-MB-468 lysates using BiP/GRP78 (Cterminus) antibody.
Immunohistochemistry analysis of paraffin-embedded Colorectal cancer using BiP/GRP78 (Cterminus) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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