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E2F1 Rabbit mAb

262152

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262152
Product Name:   Anti-E2F1 Rabbit Monoclonal Antibody
Synonyms:   E2F1; RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1
UNIPROT ID:   Q01094
Background:    The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.
Immunogen:    A synthetic peptide of human E2F1
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R03-5G3
MW:    Calculated MW: 47 kDa; Observed MW: 70 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of E2F1(green) in Hela using E2F1 antibody,and DAPI(blue)

Western blot analysis of E2F1 in MOLT4 lysates using E2F1 antibody.

Western blot analysis of E2F1 in C6, 3T3 lysates using E2F1 antibody.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using E2F1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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