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Estrogen Receptor alpha Rabbit mAb

262193

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262193
Product Name:   Anti-Estrogen Receptor alpha Rabbit Monoclonal Antibody
Synonyms:   ESR1; Era; Eralpha; Estrogen receptor; Estradiol receptor; ER-alpha; Estrogen receptor 1; NR3A1; ER; ESR; ESRA; Estrogen receptor alpha
UNIPROT ID:   P03372
Background:    ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites.
Immunogen:    A synthetic peptide of human Estrogen Receptor alpha
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP,ChIP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ChIP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R05-1E4
MW:    Calculated MW: 66 kDa; Observed MW: 66 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    NeuroscienceyyyyyEstrogen Receptor
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Estrogen Receptor alpha antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Estrogen Receptor alpha in K562, Hela lysates using Estrogen Receptor alpha antibody.

Immunocytochemistry analysis of Estrogen Receptor alpha (green) in MCF-7 using Estrogen Receptor alpha antibody,and DAPI(blue)
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