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Phospho-Histone H2A.X (Ser139) Rabbit mAb

262259

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262259
Product Name:   Anti-Phospho-Histone H2A.X (Ser139) Rabbit Monoclonal Antibody
Synonyms:   H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gamma H2A.X
UNIPROT ID:   P16104
Background:    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Immunogen:    A synthetic phosphopeptide corresponding to residues surrounding Ser139 of human gamma H2A.X
Applications:    WB,IP
Recommended Dilutions:    WB: 1/500-1/1000 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R09-4A7
MW:    Calculated MW: 15 kDa; Observed MW: 15 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of gamma H2A.X (Phospho-Ser139) in Jurkat, THP-1, C6, 3T3 lysates using gamma H2A.X (Phospho-Ser139) antibody.

Western blot analysis of gamma H2A.X (Phospho-Ser139) in HepG2 lysates using Phospho-Histone H2A.X (Ser139) antibody.
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