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Phospho-HSF1 (Ser326) Rabbit mAb

262365

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262365
Product Name:   Anti-Phospho-HSF1 (Ser326) Rabbit Monoclonal Antibody
Synonyms:   HSF1; HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1
UNIPROT ID:   Q00613
Background:    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Immunogen:    A synthetic phosphopeptide corresponding to residues surrounding Ser326 of human HSF1
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R08-4I1
MW:    Calculated MW: 57 kDa; Observed MW: 82 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Tags & Cell Markers
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of HSF1 (Phospho-Ser326)(green) in Hela using HSF1 (Phospho-Ser326) antibody,and DAPI(blue)

Western blot analysis of Phospho-HSF1 (Ser326) in K562, Hela lysates using Phospho-HSF1 (Ser326) antibody.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using HSF1 (Phospho-Ser326) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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