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KIF5B Rabbit mAb

262447

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262447
Product Name:   Anti-KIF5B Rabbit Monoclonal Antibody
Synonyms:   KNS; KINH; KNS1; UKHC; HEL-S-61
UNIPROT ID:   P33176
Background:    Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner . Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes (PubMed:20386726). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation .
Immunogen:    A synthetic peptide of human KIF5B
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R04-6G8
MW:    Calculated MW: 110 kDa; Observed MW: 110 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using KIF5B antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of KIF5B in mouse brain, rat brain lysates using KIF5B antibody.

Western blot analysis of KIF5B in Hela, MCF-7, U251MG, K562 lysates using KIF5B antibody.

Immunocytochemistry analysis of KIF5B (green) in Hela using KIF5B antibody,and DAPI(blue).
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