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Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Rabbit mAb

262669

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262669
Product Name:   Anti-Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Rabbit Monoclonal Antibody
Synonyms:   PAK3; OPHN3; Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3
UNIPROT ID:   O75914
Background:    PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. PAK3 forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets.
Immunogen:    A synthetic phosphopeptide corresponding to residues surrounding Ser144 of human PAK1
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R04-2A4
MW:    Calculated MW: 62 kDa; Observed MW: 62 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154)(green) in Hela using PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154) antibody,and DAPI(blue)

Western blot analysis of PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154) in C6, 3T3, Hela lysates using Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) antibody.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-PAK1(Ser144)/2(Ser141)/3 (Ser154) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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