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RNA Polymerases I/II/III Subunit ABC1 Rabbit mAb

262740

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262740
Product Name:   Anti-RNA Polymerases I/II/III Subunit ABC1 Rabbit Monoclonal Antibody
Synonyms:   RPB5; XAP4; RPABC1; hRPB25; hsRPB5
UNIPROT ID:   P19388
Background:    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process .
Immunogen:    A synthetic peptide of human POLR2E
Applications:    WB,IHC-P
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R05-8J5
MW:    Calculated MW: 25 kDa; Observed MW: 25 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using POLR2E antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of POLR2E in K562, C6, 3T3, Hela lysates using RNA Polymerases I/II/III Subunit ABC1 antibody.
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