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PP2A alpha/beta Rabbit mAb

262744

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262744
Product Name:   Anti-PP2A alpha/beta Rabbit Monoclonal Antibody
Synonyms:   PPP2CA; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C
UNIPROT ID:   P67775
Background:    PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
Immunogen:    A synthetic peptide of human PP2A alpha
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R04-4G4
MW:    Calculated MW: 36 kDa; Observed MW: 36 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat,Hamster
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PP2A alpha/beta antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of PP2A alpha/beta in C2C12, 3T3, mouse brain lysates using PP2A alpha/beta antibody.

Western blot analysis of PP2A alpha/beta in rat Brain, C6, CHO-K1, Hela lysates using PP2A alpha/beta antibody.

Immunocytochemistry analysis of PP2A alpha/beta (green) in hela using PP2A alpha/beta antibody,and DAPI(blue).
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