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Rab5 Rabbit mAb

262795

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262795
Product Name:   Anti-Rab5 Rabbit Monoclonal Antibody
Synonyms:   RAB5A; RAB5; RAS associated protein RAB5A; Ras related protein Rab 5A
UNIPROT ID:   P20339
Background:    Rab5 is a member of the Ras superfamily of small Rab GTPases. Rab5 is localized at the plasma membrane and early endosomes and functions as a key regulator of vesicular trafficking during early endocytosis (1). The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis.
Immunogen:    A synthetic peptide of human Rab5
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R01-6G5
MW:    Calculated MW: 24 kDa; Observed MW: 24 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Rab5 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunocytochemistry analysis of Rab5 (green) in HT-1080 using Rab5 antibody,and DAPI(blue).

Western blot analysis of Rab5 in K562, C6, 3T3, Hela lysates using Rab5 antibody.
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