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Raf1 Rabbit mAb

262807

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262807
Product Name:   Anti-Raf1 Rabbit Monoclonal Antibody
Synonyms:   RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
UNIPROT ID:   P04049
Background:    Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.
Immunogen:    A synthetic peptide of human Raf1
Applications:    WB,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R05-9B1
MW:    Calculated MW: 73 kDa; Observed MW: 73 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of Raf1 (green) in MCF-7 using Raf1 antibody,and DAPI(blue)

Western blot analysis of Raf1 in C6, 3T3, Hela lysates using Raf1 antibody.
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