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SMYD3 Rabbit mAb

262915

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262915
Product Name:   Anti-SMYD3 Rabbit Monoclonal Antibody
Synonyms:   KMT3E; ZMYND1; ZNFN3A1; bA74P14.1
UNIPROT ID:   Q9H7B4
Background:    Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
Immunogen:    A synthetic peptide of human SMYD3
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R03-2I7
MW:    Calculated MW: 49 kDa; Observed MW: 49 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using SMYD3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunocytochemistry analysis of SMYD3 (green) in K562 using SMYD3 antibody,and DAPI(blue).

Western blot analysis of SMYD3 in Jurkat, Hela lysates using SMYD3 antibody.
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