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Src Rabbit mAb

262934

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262934
Product Name:   Anti-Src Rabbit Monoclonal Antibody
Synonyms:   SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src
UNIPROT ID:   P12931
Background:    Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
Immunogen:    A synthetic peptide of human Src
Applications:    WB,IHC-P
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R05-7H8
MW:    Calculated MW: 60 kDa; Observed MW: 60 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Src antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Src in SH-SY5Y, U87-MG lysates using Src antibody.

Western blot analysis of Src in C6, 3T3 lysates using Src antibody.
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