TRIM25 Rabbit mAb

263025

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: N263025

Product Name: Anti-TRIM25 Rabbit Monoclonal Antibody

Synonyms: TRIM25; EFP; RNF147; ZNF147; E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147

UNIPROT ID: Q14258

Background: Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection.

Immunogen: A synthetic peptide of human TRIM25

Applications: WB,ICC/IF,IP

Recommended Dilutions: WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20

Host Species: Rabbit

Clonality: Rabbit Monoclonal

Clone ID: R06-1H2

MW: Calculated MW: 71 kDa; Observed MW: 71 kDa

Isotype: IgG

Purification: Affinity Purified

Species Reactivity: Human,Rat

Conjugation: Unconjugated

Modification: Unmodified

Constituents: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Research Areas: Cell Biology

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of TRIM25/EFP (green) in MCF-7 using TRIM25/EFP antibody,and DAPI(blue)

Western blot analysis of TRIM25/EFP in K562, rat Brain, C6, Hela lysates using TRIM25 antibody.

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