VPS4A Rabbit mAb

263079

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: N263079

Product Name: Anti-VPS4A Rabbit Monoclonal Antibody

Synonyms: vacuolar protein sorting 4 homolog A; SKD1; SKD2; VPS4; SKD1A; VPS4-1

UNIPROT ID: Q9UN37

Background: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Immunogen: A synthetic peptide of human VPS4a

Applications: WB,IHC-F,IHC-P,ICC/IF

Recommended Dilutions: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Host Species: Rabbit

Clonality: Rabbit Monoclonal

Clone ID: R01-9H9

MW: Calculated MW: 49 kDa; Observed MW: 49 kDa

Isotype: IgG

Purification: Affinity Purified

Species Reactivity: Human,Mouse,Rat

Conjugation: Unconjugated

Modification: Unmodified

Constituents: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Research Areas: Cell Biology

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of VPS4a(green) in Hela using VPS4a antibody,and DAPI(blue)	Western blot analysis of VPS4a in K562, rat Brain, C6, 3T3 lysates using VPS4A antibody.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using VPS4a antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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