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RNA Polymerase II Subunit B1 Rabbit mAb

263136

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N263136
Product Name:   Anti-RNA Polymerase II Subunit B1 Rabbit Monoclonal Antibody
Synonyms:   POLR2A; POLR2; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1
UNIPROT ID:   P24928
Background:    During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.
Immunogen:    A synthetic peptide of human RNA polymerase II CTD repeat YSPTSPS
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R06-8G7
MW:    Calculated MW: 217 kDa; Observed MW: 250 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using RNA polymerase II CTD repeat YSPTSPS antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunocytochemistry analysis of RNA Polymerase II Subunit B1 (green) in CEM using RNA Polymerase II Subunit B1 antibody,and DAPI(blue).

Western blot analysis of RNA polymerase II CTD repeat YSPTSPS in Hela, A549, MCF-7, U251, HL-60 lysates using RNA polymerase II CTD repeat YSPTSPS antibody.
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