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TriMethyl-Histone H3 (Lys27) Rabbit mAb

263146

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N263146
Product Name:   Anti-TriMethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody
Synonyms:   H3K27me3; H3 histone; HIST1H3A; Histone cluster 1; H3a
UNIPROT ID:   P68431
Background:    H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Immunogen:    A synthetic methyl-peptide corresponding to residues surrounding Lys27 of human Histone H3
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP,ChIP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ChIP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R01-8I4
MW:    Calculated MW: 15 kDa; Observed MW: 15 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Rat
Conjugation:    Unconjugated
Modification:    Methylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    EpigeneticsyyyyyHistone methylation
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of TriMethyl-Histone H3 (Lys27) (green) in Hela using TriMethyl-Histone H3 (Lys27) antibody,and DAPI(blue)

Western blot analysis of Histone H3 (tri methylLys27) in C6, Jurkat lysates using TriMethyl-Histone H3 (Lys27) antibody.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using TriMethyl-Histone H3 (Lys27) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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