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Acetyl-Histone H3 (Lys27) Rabbit mAb

263147

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N263147
Product Name:   Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody
Synonyms:   H3K27ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J
UNIPROT ID:   P68431
Background:    H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Immunogen:    A synthetic acetyl-peptide corresponding to residues surrounding Lys27 of human Histone H3
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP,ChIP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ChIP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R08-4A0
MW:    Calculated MW: 15 kDa; Observed MW: 15 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Acetylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Histone H3 (acetylLys27) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Histone H3 (acetylLys27) in Hela, A549, HL-60 lysates using Acetyl-Histone H3 (Lys27) antibody.

Immunocytochemistry analysis of Histone H3 (acetylLys27) (green) in hela using Histone H3 (acetylLys27) antibody,and DAPI(blue)
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