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Phospho-Histone H3 (Ser10/Thr11) Rabbit mAb

263168

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N263168
Product Name:   Anti-Phospho-Histone H3 (Ser10/Thr11) Rabbit Monoclonal Antibody
Synonyms:   H3 histone; family 3A; H3 histone; family 3B (H3.3B); H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3
UNIPROT ID:   P68431
Background:    H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Immunogen:    A synthetic phosphopeptide corresponding to residues surrounding Ser10/Thr11 of human Histone H3
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R08-6C4
MW:    Calculated MW: 15 kDa; Observed MW: 15 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    EpigeneticsyyyyyHistone phosphorylation
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of Histone H3 (Phospho-Ser10/Thr11) in Hela, A549, HL-60, C6 lysates using Histone H3 (Phospho-Ser10/Thr11) antibody

Immunocytochemistry analysis of Phospho-Histone H3 (Ser10/Thr11) (green) in Hela using Phospho-Histone H3 (Ser10/Thr11) antibody,and DAPI(blue).

Western blot analysis of Phospho-Histone H3 (Ser10/Thr11) in 3T3 lysates using Phospho-Histone H3 (Ser10/Thr11)antibody.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Histone H3 (Phospho-Ser10/Thr11) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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