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5T4 Rabbit mAb

263203

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N263203
Product Name:   Anti-5T4 Rabbit Monoclonal Antibody
Synonyms:   5T4; 5T4AG; M6P1; TPBG; 5T4 oncofetal antigen; Trophoblast glycoprotein
UNIPROT ID:   Q13641
Background:    5T4 is a 72 kDa transmembrane glycoprotein that exhibits restricted expression in human and mouse adult tissues, is upregulated on many carcinomas and tumour expression correlates with poorer clinical outcome in ovarian, gastric and colorectal cancers. mES cells lack cell surface expression of the 5T4 antigen, and both protein and mRNA are rapidly upregulated following induction of differentiation. This proteins expression correlates with downregulation of OCT-4 and Tra-1–60.
Immunogen:    A synthetic peptide of human 5T4
Applications:    WB,IP
Recommended Dilutions:    WB: 1/500-1/1000 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R05-3A0
MW:    Calculated MW: 46 kDa; Observed MW: 78 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of 5T4 in 293T, C2C12 lysates using 5T4 antibody.

Western blot analysis of 5T4 in mouse brain, rat brain lysates using 5T4 antibody.
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