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Keap1 Rabbit mAb

263393

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N263393
Product Name:   Anti-Keap1 Rabbit Monoclonal Antibody
Synonyms:   INrf2; KLHL19
UNIPROT ID:   Q14145
Background:    This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]
Immunogen:    Recombinant protein of human Keap1
Applications:    WB,IHC-P
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R02-1H7
MW:    Calculated MW: 70 kDa; Observed MW: 60-64 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Keap1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Keap1 in Raw264.7 lysates using Keap1 antibody.

Western blot analysis of Keap1 in Hela, A549, C2C12 lysates using Keap1 antibody.
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