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TATA Box Binding Protein Rabbit mAb

263571

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N263571
Product Name:   Anti-TATA Box Binding Protein Rabbit Monoclonal Antibody
Synonyms:   TBP; GTF2D1; TF2D; TFIID; TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit
UNIPROT ID:   P20226
Background:    As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This protein is not suitable for samples where the nuclear envelope has been removed.
Immunogen:    A synthetic peptide of human TATA binding protein TBP
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R04-6D1
MW:    Calculated MW: 38 kDa; Observed MW: 38 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human tonsil using TATA binding protein TBP antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of TATA binding protein TBP in 3T3 lysates using TATA Box Binding Protein antibody.

Western blot analysis of TATA binding protein TBP in Hela, A549, HL-60, U2OS lysates using TATA binding protein TBP antibody.

Immunocytochemistry analysis of TATA binding protein TBP (green) in hela using TATA binding protein TBP antibody,and DAPI(blue).
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