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Mammalian Protein Service

Mammalian Protein Expression Service

We are an expert on CHO and HEK293 cell expression.  We can meet your special needs such as serum-free culture, tag excision, endotoxin removal, and mass spectrometry analysis.
Mammalian cells have become the preferred host cells in the field of biopharmaceuticals. Mammalian cell expression systems can introduce proper protein folding  and carry out post-translational modifications, which are essential for full biological activity. The expressed recombinant proteins are closest to the human conformation and have the same biological activity as natural proteins. Heterologous proteins expressed by mammalian cells have the incomparable advantages of specific prokaryotic expression systems and eukaryotic expression systems such as yeast and insect cells in terms of activity, which are closer to natural proteins. Therefore, mammalian cell expression systems have been widely used in the development and production of therapeutic recombinant monoclonal antibody drugs and protein drugs. We has established a mature mammalian protein expression system and purification service platform, providing expression and purification services of mammalian protein expression system to help you quickly obtain high-quality protein.
 
Advantage
   
 
 
Highlights Summary
High activity and high success rate Proteins expressed in mammalian cells can achieve complex post-translational modifications.
Multiple expression system options Transient transfection expression
Stable transfection expression
Recombinant antibody expression,and preparation
One-stop Service Design and optimize gene
Optimize protein expression conditions
Purify protein and post processing
Manage data and report.
Stable Cell Line Construction
   
 
 
Step Description Timeline
Vector Design Conducts bioinformatic analysis on target(s) and recommends an expression strategy to each client. 1 Week
Plasmid Construction Gene optimization and synthesis, Vector construction 2-3 Weeks
Identification of polyclonal stable cell lines Vector transformation, screening and identification
identification of positive cell clones
2-3 Weeks
Vector transformation, screening and identification
identification of positive cell clones
Cell subclonal screening to screen out monoclonal cell lines with high expression levels and stable performance 3-4 Weeks
Stable cell line quality test Perform performance verification according to customer requirements, such as protein activity, expression level, cell passage stability, etc. 1-2 Weeks

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