Rheb-GTP

26910

Size：30 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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Rheb-GTP

Cat. #: 26910

Size: 30 µL

Gene Symbol: RHEB

Description: Anti-Rheb-GTP Monoclonal Antibody

Background: Small GTPases are a super-family of cellular signaling regulators. Rheb is a member of the Ras-superfamily GTPases. Rheb has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related (ATR).

Immunogen: Recombinant full-length protein of active Rheb.

Applications: IP, IHC, IF

Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgM

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-Rheb-GTP monoclonal antibody recognizes active Rheb of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze/thaw cycles

Immunoprecipitation/Western blot:

Rheb activation assay. Purified Rheb proteins were immunoprecipitated after treated with GDP (lane 1) or GTP_γS (lane 2). Immunoprecipitation was done with the anti-Rheb-GTP monoclonal antibody (Cat. No. 26910). Immunoblot was with an anti-Rheb rabbit polyclonal antibody (Cat. No. 21098).

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Rheb-GTP

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