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Ral-GTP

26913

Size:30 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:

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Ral-GTP

Cat. #:   26913
Size:  30 µL
Gene Symbol:  Ral
Description:   Anti-Ral-GTP Mouse Monoclonal Antibody
Background:   The Ras-like small G proteins, RalA and RalB, are important components of Ras signaling pathways, implicated in the initiation and maintenance of tumorigenic transformation, as well as vesicle transport, apoptosis, transcription, cell migration, and cell proliferation.
Immunogen:   Recombinant full length protein of active RalB.
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:   
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Ral-GTP monoclonal antibody recognizes active RalA and RalB of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:  
Ral activation assay. Purified RalB proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Ral-GTP monoclonal antibody (Cat. No. 26913). Immunoblot was with an anti-RalB rabbit polyclonal antibody (Cat. No. 21033).
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