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Rab4A-GTP

26921

Size:30 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:

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Rab4-GTP

Cat. #:   26921
Size:  30 µL
Gene Symbol:  RAB4
Description:   Anti-Rab4-GTP Monoclonal Antibody
Background:   Rab-4 belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20, RUFY1, SGSM1, SGSM2 and SGSM3. It participates in protein transport and in vesicular traffic.
Immunogen:   Recombinant full length protein of active Rab4A.
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:   
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Rab4-GTP monoclonal antibody recognizes active Rab4 from vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:  
Rab4A Activation Assay. Purified GST-tagged Rab4A proteins (Cat. #10130) were immunoprecipitated with the anti-Rab4-GTP monoclonal antibody (Cat. No. 26921) after treated with GDP (lane 1) or GTPγ S (lane 2), and was blotted with anti-Rab4A polyclonal antibody (Cat. No. 21067). Input control is shown in the bottom panel.
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