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z-GTP

26908

Size:30 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:

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z-GTP
Cat. #:   26908
Size:  30 µL
Gene Symbol:  Gnaz
Description:   Anti-Gαz-GTP Mouse Monoclonal Antibody
Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαz represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαz proteins are being explored.
Immunogen:   Recombinant full length protein of active Gαz
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:   IP: 1 µg for 1~2 mg total cellular proteinsIHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Gαz-GTP monoclonal antibody recognizes active Gαz of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:  z activation assay. Purified Gαz proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-Gαz-GTP monoclonal antibody (Cat. No. 26908). Immunoblot was with an anti-Gαz polyclonal antibody.
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