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Size:30 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:
Cat. #: 26906 |
Size: |
Gene Symbol: Gnas |
Description: Anti-Gαs-GTP Mouse Monoclonal Antibody |
Background: Heterotrimeric G proteins are critical cellular signal transducers. Gαs represents one sub-family of G proteins that could mediate the stimulation of adenylyl cyclases. |
Immunogen: Recombinant full length protein of active Gαs |
Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
Concentration: 1 mg/ml |
Host Species: |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Anti-active Gαs antibody recognizes active Gαs of vertebrates |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot: Gαs Activation Assay. Purified Gαs proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti Gαs-GTP monoclonal antibody (Cat. No. 26906). Immunoblot was with an anti- Gαs monoclonal antibody (Cat. No. 26006). |
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Bioactive Transmembrane Proteins Antibodies for Transmembrane Proteins G Protein | GTPase