Arl1-GTP

26924

Size：30 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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Arl1-GTP

Cat. #: 26924

Size: 30 µL

Gene Symbol: Arl1

Description: Anti-Arl1-GTP Mouse Monoclonal Antibody

Background: Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Golgi-localized proteins golgin-97 and golgin-245 have been shown as Arl1 effectors. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. In Drosophila zygotes, mutation of Arl1 is lethal.

Immunogen: Recombinant Arl1 protein (active form)

Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)

Recommended Dilutions:
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Arl1 antibody recognizes active Arl1-GTP from vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation/Western blot:
Arl1 Activation Assay.

Purified GST-tagged Arl1 proteins (Cat. #10136) were immunoprecipitated with the anti-active Arl1 monoclonal antibody (Cat. # 26924) after treated with GDP (lane 1) or GTP_γS (lane 2), and was blotted with anti- Arl1 monoclonal antibody (Cat. #26056). Arl1 protein input control is shown in the bottom panel.

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Arl1-GTP

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