Arf6-GTP

26918

Size：30 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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Arf6-GTP

Cat. #: 26918

Size: 30 µL

Gene Symbol: ARF6

Description: Anti-Arf6-GTP Monoclonal Antibody

Background: ARF6 (ADP-ribosylation factor 6) is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and functions as a regulatory molecule of phagocytosis.

Immunogen: Recombinant full length protein of active Arf6.

Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)

Recommended Dilutions:
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-Arf6-GTP monoclonal antibody recognizes active Arf6 from vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation/Western blot:

Arf6 activation assay.

Purified full-length Arf6 proteins were immunoprecipitated after treated with GDP (lane left) or GTP_γS (lane right). Immunoprecipitation was done with the anti-Arf6-GTP monoclonal antibody (Cat. No. 26918). Immunoblot was with an anti-Arf6 polyclonal antibody (Cat. No. 21032).

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Arf6-GTP

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