Gαi mAb

26003

Size：100 μl Price：$ 239 Brand：NewEast Place of Origin：USA Immunogen：

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Gα_i Monoclonal Antibody

Cat. #: 26003

Gene Symbol: Gnai

Description: Anti-Gα_i Mouse Monoclonal Antibody

Background: Heterotrimeric G proteins are critical cellular signal transducers. Gai represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gai proteins are being explored.

Immunogen: Recombinant full length protein of Gα_i1

Applications: ELISA, WB, IHC

Recommended Dilutions: ELISA 1:1000-1:5000,WB 1:1000-1:2000,IHC 1:100-1:200

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Gα_i antibody recognizes Gα_i1, Gα_i2, and Gα_i3 of vertebrates

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Western blot:

Western blot analysis of Gi proteins. Gα_i1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blots. The monoclonal anti-Gα_i antibody was pre-incubated without (lane 1) or with (lane 2) the antigen protein (Gα_i1) to show the specificity.

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