Email Us：info@neweastbio.com
Call Us：(+1) 610-945-2007
Email Us：info@neweastbio.com
Call Us：(+1) 610-945-2007
Size：100 μl
Price：$ 495
Brand：NewEast 
Place of Origin：USA
Immunogen：
| Cat. #:: 26084 |
| Gene Symbol: IDH2 |
| Description: Anti-IDH2(R172H) Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-172 in IDH2 to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| Immunogen: A synthetic peptide from the internal region of human IDH2 which includes the mutation of R172H. |
| Applications: ELISA, WB, IHC, IF |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 IF: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-IDH2(R172H) antibody recognizes IDH2(R172H) of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. |
Western blot analysis of recombinant IDH2(R172H) and wild type IDH2 proteins.
Purified his-tagged IDH2(R172H) protein (Cat. # 10201) and wild type IDH2 protein (Cat. # 10200) were blotted with anti-IDH2(R172H) monoclonal antibody (Cat. # 26084) (left panel). Proteins loading were blotted with anti-IDH2 monoclonal antibody (right panel).
Immunofluorescence:
Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172H) antibody.
HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R172H) plasmid (right column), then fixed and stained with Anti-IDH2(R172H) monoclonal antibody (Cat. #26084).
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