EGFR(vIII)

26176

Size：100 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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EGFR(vIII)

Cat. #: 26176

Gene Symbol: EGFR/HER1/ERBB1

Description: Anti-EGFR(vIII) Mouse Monoclonal Antibody

Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (NSCLC).

Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of vIII, human origin.

Applications: ELISA, WB, IF, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:1000-1:5000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes EGFR(vIII), but not wild type EGFR from vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Western blot:

Western blot analysis of recombinant EGFR(vIII) and wild type proteins.

Purified His-tagged EGFR(vIII) protein (partial, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-EGFR(vIII) mouse monoclonal antibody (Cat. # 26176).

Immunofluorescence:

Immunofluorescence of cells expressing EGFR proteins with anti-EGFR(vIII) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(vIII) plasmid (right column), then fixed and stained with anti-EGFR(vIII) monoclonal antibody (Cat. #26176).

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EGFR(vIII)

IDH2(R172G)

IDH2(R172W)

IDH2(R172K)

IDH2(R172S)

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