Email Us：info@neweastbio.com
Call Us：(+1) 610-945-2007
Email Us：info@neweastbio.com
Call Us：(+1) 610-945-2007
Size：100 μl
Price：$ 495
Brand：NewEast 
Place of Origin：USA
Immunogen：
EGFR(V769L)
| Cat. #: 26121 |
| Gene Symbol: EGFR/HER1/ERBB1 |
| Description: Anti-EGFR(V769L) Mouse Monoclonal Antibody |
| Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). |
| Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of V769L, human origin. |
| Tested Applications: ELISA, WB, If, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes EGFR(V769L), but not wild type EGFR from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. |
Western blot:
Western blot analysis of recombinant EGFR(V769L) and wild type proteins.
Purified His-tagged EGFR(V769L) protein (amino acids 702-987, lane 2, Cat. #10239) and corresponding wild type protein (lane 1, Cat. #10210) were blotted with anti EGFR(V769L) mouse monoclonal antibody (Cat. #26121). Proteins loading (bottom) were blotted with anti-His-tag monoclonal antibody (Cat. #28004).
Immunofluorescence:
Immunofluorescence of cells expressing EGFR proteins with anti EGFR(V769L) antibody.
HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP EGFR(V769L) plasmid (right column), then fixed and stained with anti- EGFR(V769L) monoclonal antibody (Cat. # 26121).
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Bioactive Transmembrane Proteins Antibodies for Transmembrane Proteins G Protein | GTPase
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