β-Catenin(E53K)

26167

Size：100 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

Add to cart

Download

Datasheet

MSDS

Product Description Reference citations(0) Related Products

β-Catenin(E53K)

Cat. #: 26167

Gene Symbol: CTNNB1, CTNNB

Description: Anti-β-catenin(E53K) Mouse Monoclonal Antibody

Background: Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.

Immunogen: A synthetic peptide from the internal region of β-catenin which includes the mutation of E53K, human origin.

Applications: ELISA, WB, IF, IHC

Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:50-1:100

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes β-catenin(E53K), but not wild type β-catenin protein from vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Western blot:

Western blot analysis of recombinant β-catenin(E53K) and wild type proteins. Purified His-tagged β-catenin(E53K) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-β-catenin(E53K) mouse monoclonal antibody (Cat. #26167). Immunofluorescence:

Immunofluorescence of cells expressing β-catenin proteins with anti-β-catenin(E53K) antibody. HEK293T cells were transfected with pCDNA3-GFP- β-catenin (WT) plasmid (left column) or pCDNA3-GFP-β-catenin(E53K) plasmid (right column), then fixed and stained with Anti-β-catenin (E53K) monoclonal antibody (Cat. #26167).

Reference citations

No data available

Product Targets

Product Categories

β-Catenin(E53K)

IDH2(R172G)

IDH2(R172W)

IDH2(R172K)

IDH2(R172S)

Contant Us