FBXW7(R479L)

26347

Size：100 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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FBXW7(R479L)

Cat. #: 26347

Gene Symbol: FBW6; FBW7; FBXW7; FBX30; CDC4; SEL10

Description: Anti-FBXW7(R479L) Mouse Monoclonal Antibody

Background: F-box/WD repeat-containing protein 7 is a protein that in humans is encoded by the FBXW7 gene. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Mutations in this gene are detected in ovarian, breast and colorectal cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers.

Immunogen: A synthetic peptide from the internal region of FBXW7 which includes the mutation of R479L, human origin.

Applications: ELISA, WB

Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes R479L mutant, but not wild type FBXW7 of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Western blot:

Western blot analysis of recombinant FBXW7(R479L) and wild type proteins. Purified His-tagged FBXW7(R479L) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-FBXW7(R479L) monoclonal antibody (Cat. #26347). Immunofluorescence:

Immunofluorescence of cells expressing FBXW7 proteins with Anti-FBXW7(R479L) antibody. HEK293T cells were transfected with pCDNA3-GFP-FBXW7 (WT) plasmid (left column) or pCDNA3-GFP-FBXW7(R479L) plasmid (right column), then fixed and stained with Anti-FBXW7(R479L) monoclonal antibody (Cat. #26347).

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FBXW7(R479L)

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