ERBB2 pAb

21202

Size：100 μl Price：$ 219 Brand：NewEast Place of Origin：USA Immunogen：

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ErbB2 Polyclonal Antibody

Cat. #: 21202

Gene Symbol: ERBB2/HER2/Neu

Description: Anti-ErbB2 Rabbit Polyclonal Antibody

Background: The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis.

Immunogen: A synthetic peptide from the central of ErbB2 protein.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:500-1:5000
WB: 1:200-1:2000
IHC: 1:20-1:200

Concentration: 0.2 mg/ml

Host Species: Rabbit

Format: Liquid

Clonality: Polyclonal

Isotype: IgG

Purity: Purified from serum

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes ErbB2 of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing.

Western blot:

Western blot analysis of ErbB2 protein. Purified full length His-tagged ErbB2 protein (Cat. #10208) was blotted with Anti-ErbB2 rabbit polyclonal antibody (Cat. #21202).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Anti-ErbB2 polyclonal antibody (Cat. #21202). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4 °C. A HRP-conjugated Goat anti-Rabbit IgG (1:50) was used as secondary antibody.

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ERBB2 pAb

IDH2(R172G)

IDH2(R172W)

IDH2(R172K)

IDH2(R172S)

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